Fluorescent Base Analogue Reveals T-HgII-T Base Pairs Have High Kinetic Stabilities That Perturb DNA Metabolism
نویسندگان
چکیده
منابع مشابه
Raman spectroscopic detection of the T-HgII-T base pair and the ionic characteristics of mercury
Developing applications for metal-mediated base pairs (metallo-base-pair) has recently become a high-priority area in nucleic acid research, and physicochemical analyses are important for designing and fine-tuning molecular devices using metallo-base-pairs. In this study, we characterized the Hg(II)-mediated T-T (T-Hg(II)-T) base pair by Raman spectroscopy, which revealed the unique physical an...
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Owing to their great potentials in genetic code extension and the development of nucleic acid-based functional nanodevices, DNA duplexes containing HgII-mediated base pairs have been extensively studied during the past 60 years. However, structural basis underlying these base pairs remains poorly understood. Herein, we present five high-resolution crystal structures including one first-time rep...
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While fluorescent analogues of the canonical nucleobases have proven to be highly valuable in a large number of applications, up until today, fluorescent DNA base analogues remain virtually inapplicable for single-molecule fluorescence experiments which require extremely bright and photostable dyes. Insight into the photodegradation processes of these fluorophores is thus a key step in the cont...
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DNA double helices comprising chimeric GNA/DNA metal-mediated base pairs have been synthesized and characterized (GNA = glycol nucleic acid). The possibility to combine different nucleic acid backbones within one metal-mediated base pair expands the applicability of metal-functionalized nucleic acids.
متن کاملChapter 9 Minor Groove Recognition of T , A Base Pairs by Benzothiophene / Pyrrole Pairs
Polyamides are designed oligomers that can bind to DNA in a sequence-specific manner with affinities comparable to those of natural DNA-binding proteins. Recently, an N-terminal 3-chloro-6-fluorobenzothiophene polyamide bound in the minor groove of DNA was co-crystallized at 1.1 Å resolution as an antiparallel dimer. In addition, antimicrobial screenings for this polyamide family and the develo...
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ژورنال
عنوان ژورنال: Journal of the American Chemical Society
سال: 2016
ISSN: 0002-7863,1520-5126
DOI: 10.1021/jacs.6b09044